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pdxT [2020-02-12 11:38:10]
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pdxT [2020-02-12 11:38:10]

pyridoxal-5-phosphate synthase (glutaminase domain)
Locus
BSU_00120
Isoelectric point
4.98
Molecular weight
21.30 kDa
Protein length
196 aa Sequence Blast
Gene length
591 bp Sequence Blast
Function
pyridoxal-5-phosphate biosynthesis
Product
pyridoxal-5-phosphate synthase (glutaminase domain)
Essential
no
E.C.
4.3.3.6
Synonyms
yaaE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of pyridoxal phosphate
  • Information processing → Genetics → Newly identified competence genes

    • Gene

    Coordinates
    19,968 20,558

    Phenotypes of a mutant

  • inactivation of pdxT reduces sporulation efficiency to 1.2% that of wild type cells; delayed entry into sporulation PubMed
  • poor growth PubMed
  • non-transformable PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS. (according to UniProt)
  • aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine --> H+ + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate (according to UniProt)

    • Protein family

  • Glutaminase PdxT/SNO family (single member, according to UniProt)

    • Structure

  • 2NV2 PdxS-PdxT complex PubMed
  • 2NV0 (PdxT) PubMed

    • Localization

  • cytosol (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    dacA-pdxS-pdxT-serS
    Description
    PubMed

    Regulation

  • the leader mRNA is processed upstream of dacA by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

    • Additional information

  • PhoP∼P binds to the promoter region, but regulation has not been explored PubMed
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    Genes
    pdxS-pdxT
    Description
    PubMed

    Regulatory mechanism

  • Spo0A: repression, PubMed, in Spo0A regulon

    • Regulation

  • negatively controlled by Spo0A PubMed
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    Genes
    pdxS-pdxT-serS
    Description
    PubMed
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    Biological materials

    Mutant

  • BKE00120 (pdxT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCAGCAGCGCTCCTAT, downstream forward: _UP4_TAAAACAGTTGAAAGCTGTG
  • BKK00120 (pdxT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCAGCAGCGCTCCTAT, downstream forward: _UP4_TAAAACAGTTGAAAGCTGTG
  • BV604 ((pdxS-pdxT)::tet), available in Fabian Commichau's lab, PubMed

    • References

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